PUBLICATIONS
2023
"IMPORTANT ENOUGH TO SHOW THE WORLD": USING AUTHENTIC RESEARCH OPPORTUNITIES AND MICROPUBLICATIONS TO BUILD STUDENTS' SCIENCE IDENTITIES.
Rubenstein, LD, Woodruff KA, Taylor AM, Olesen JB, Smaldino PJ, Rubenstein EM. 2023. “'Important enough to the show the world:' Using Authentic Research Opportunities and Micropublications to Build Students' Science Identities” bioRxiv, published online (doi: https://doi.org/10.1101/2023.08.17.553701).
2023
CHARACTERIZATION OF ENDOPLASMIC RETICULUM-ASSOCIATED DEGRADATION IN THE HUMAN FUNGAL PATHOGEN CANDIDA ALBICANS
Doss EM, Moore JM, Harman BH, Doud EH, Rubenstein EM†*, Bernstein DA*. 2023. “Characterization of Endoplasmic Reticulum-Associated Degradation in the human fungal pathogen Candida albicans.” PeerJ 11:e15897. †, lead contact. *, co-corresponding authors.
2023
LIPID BIOSYNTHESIS PERTURBATION IMPAIRS ENDOPLASMIC RETICULUM-ASSOCIATED DEGRADATION
Turk SM*, Indovina CJ*, Miller JM, Overton DL, Runnebohm AM, Tragesser-Tiña ME, Gosser SK, Orchard CJ, Doss EM, Richards KA, Irelan CB, Daraghmi MM, Bailey CG, Niekamp JM, Claypool KP, Engle SM, Buchanan BW, Woodruff KA, Olesen JB, Smaldino PJ, Rubenstein EM. 2023. Lipid Biosynthesis Perturbation Impairs Endoplasmic Reticulum-Associated Degradation. Journal of Biological Chemistry. published online (doi: https://doi.org/10.1016/j.jbc.2023.104939), *, equal contributions.
2023
MACRO-ER-PHAGY RECEPTORS ATG39P AND ATG40P CONFER RESISTANCE TO AMINOGLYCOSIDE HYGROMYCIN B IN S. CEREVISIAE
Daraghmi MM*, Miller JM*, Bailey CG, Doss EM, Kalinski AL, Smaldino PJ, Rubenstein EM. 2023. Macro-ER-phagy receptors Atg39p and Atg40p confer resistance to aminoglycoside hygromycin B in S. cerevisiae. microPublication Biology, published online (https://doi.org/10.17912/micropub.biology.000738), *, equal contributions.
2022
CLEARING THE WAY: HOW CELLS UNCLOG CLOGGED CHANNELS
Rubenstein EM. 2022. Clearing the Way: How Cells Unclog Clogged Channels. Proceedings of the Indiana Academy of Science, 130 (179-185).
2022
APC/C CDH1P AND SLX5P/SLX8P UBIQUITIN LIGASES CONFER RESISTANCE TO AMINOGLYCOSIDE HYGROMYCIN B IN SACCHAROMYCES CEREVISIAE
Doss EM, Tragesser-Tiña ME, Huang Y, Smaldino PJ, True JD, Kalinski AL, Rubenstein EM. 2022. APC/CCdh1p and Slx5p/Slx8p ubiquitin ligases confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae. microPublication Biology. 10.17912/micropub.biology.000547.
2021
INNER NUCLEAR MEMBRANE ASI UBIQUITIN LIGASE CATALYTIC SUBUNITS ASI1P AND ASI3P, BUT NOT ASI2P, CONFER RESISTANCE TO AMINOGLYCOSIDE HYGROMYCIN B IN SACCHAROMYCES CEREVISIAE
Woodruff KA, Richards KA, Evans MD, Scott AR, Voas BM, Irelan CB, Olesen JB, Smaldino PJ, Rubenstein EM. Inner Nuclear Membrane Asi Ubiquitin Ligase Catalytic Subunits Asi1p and Asi3p, but not Asi2p, confer resistance to aminoglycoside hygromycin B in Saccharomyces cerevisiae. 2021. microPublublication Biology. 10.17912/micropub.biology.000403. doi: 10.17912/micropub.biology.000403. PMID: 34095778; PMCID: PMC8170509.
2020
OVERLAPPING FUNCTION OF HRD1 AND STE24 IN TRANSLOCON QUALITY CONTROL PROVIDES ROBUST CHANNEL SURVEILLANCE
Runnebohm AM*, Richards KA*, Broshar Irelan CL, Turk SM, Vitali HE, Indovina CJ, and Rubenstein EM. 2020. Overlapping function of Hrd1 and Ste24 in translocon quality control provides robust channel surveillance. Journal of Biological Chemistry. DOI: 10.1074/jbc.AC120.016191, *, equal contributions.
2020
G‑QUADRUPLEX HELICASE DHX36/G4R1 ENGAGES NUCLEAR LAMINA
PROTEINS IN QUIESCENT BREAST CANCER CELLS
Richardson, AE, Zentz ZA, Chambers AE, Sandwith SN, Reisinger MA, Saunders DW, Tompkins JD, Riggs SD, Routh ED, Rubenstein EM, Smaldino MA, Vaughn JP, Haney RA, and Smaldino PJ. 2020. G-Quadruplex Helicase DHX36/G4R1 Engages Nuclear Lamina Proteins in Quiescent Breast Cancer Cells. ACS Omega. 5:24916-24926
2020
ENDOPLASMIC RETICULUM STRESS-REGULATED DEGRADATION OF A TRANSLOCON-ASSOCIATED PROTEIN IS INDEPENDENT OF INTEGRATED STRESS RESPONSE TRANSCRIPTION FACTOR GCN4P
Richards KA, Rubenstein EM. 2020. Endoplasmic reticulum stress-regulated degradation of a translocon-associated protein is independent of integrated stress response transcription factor Gcn4p. microPublication Biology, published online (https://doi.org/10.17912/micropub.biology.000239).
2020
GLUCOSE CONCENTRATION DOES NOT AFFECT DEGRADATION OF A PROTEIN THAT ABERRANTLY ENGAGES THE ENDOPLASMIC RETICULUM TRANSLOCON
Broshar CL, Rubenstein EM. 2020. Glucose concentration does not affect degradation of a protein that aberrantly engages the endoplasmic reticulum translocon. microPublication Biology, published online (https://doi.org/10.17912/micropub.biology.000248).
2020
LOSS OF PROTEIN QUALITY CONTROL GENE UBR1 SENSITIZES SACCHAROMYCES CEREVISIAE TO THE AMINOGLYCOSIDE HYGROMYCIN B
Runnebohm AM*, Evans MD*, Richardson AE, Turk SM, Olesen JB, Smaldino PJ, Rubenstein EM. 2020. Loss of protein quality control gene UBR1 sensitizes Saccharomyces cerevisiae to the aminoglycoside hygromycin B. Fine Focus, *, equal contributions.
2019
ENDOPLASMIC RETICULUM STRESS DIFFERENTIALLY INHIBITS ENDOPLASMIC RETICULUM AND INNER NUCLEAR MEMBRANE PROTEIN QUALITY CONTROL DEGRADATION PATHWAYS
Buchanan BW, Mehrtash AB*, Broshar CL*, Runnebohm AM, Snow BJ, Scanameo LN, Hochstrasser M, Rubenstein EM. 2019. Endoplasmic reticulum stress differentially inhibits endoplasmic reticulum and inner nuclear membrane protein quality control degradation pathways. Journal of Biological Chemistry, 294 (19814–19830), *, equal contributions.
2019
TOM1 CONFERS RESISTANCE TO THE AMINOGLYCOSIDE HYGROMYCIN B IN SACCHAROMYCES CEREVISIAE
Niekamp JM, Evans MD, Scott AR, Smaldino PJ, Rubenstein EM. 2019. TOM1 confers resistance to the aminoglycoside hygromycin B in Saccharomyces cerevisiae. microPublication Biology, published online (https://www.micropublication.org/journals/biology/micropub-biology-000193/).
2018
HOT AND TOXIC: TEMPERATURE REGULATES MICROCYSTIN RELEASE FROM CYANOBACTERIA
Walls JT, Wyatt KH, Doll JC, Rubenstein EM, Rober AR. 2018. Hot and toxic: Temperature regulates microcystin release from cyanobacteria. Science of the Total Environment 610–611 (786–795).
2017
ACETYLATION OF N-TERMINUS AND TWO INTERNAL AMINO ACIDS IS DISPENSABLE FOR DEGRADATION OF A PROTEIN THAT ABERRANTLY ENGAGES THE ENDOPLASMIC RETICULUM TRANSLOCON
Engle SM, Crowder JJ, Watts SG, Indovina CJ, Coffey SZ, Rubenstein EM. 2017. Acetylation of N-terminus and two internal amino acids is dispensable for degradation of a protein that aberrantly engages the endoplasmic reticulum translocon. PeerJ 5:e3728.
2016
CYCLOHEXIMIDE CHASE ANALYSIS OF PROTEIN DEGRADATION IN SACCHAROMYCES CEREVISIAE
Buchanan BW, Lloyd ME, Engle SM, Rubenstein EM. 2016. Cycloheximide chase analysis of protein degradation in Saccharomyces cerevisiae. Journal of Visualized Experiments 110 (e53975).
2016
T24 HRAS TRANSFORMED NIH/3T3 MOUSE CELLS (GHRAST-NIH/3T3) IN SERIAL TUMORIGENIC PASSAGES GIVE RISE TO INCREASINGLY AGGRESSIVE TUMORIGENIC CELL LINES T1-A AND T2-A AND METASTATIC CELL LINES T3-HA AND T4-PA
Ray DB, Merrill GA, Brenner FJ, Lytle LL, Lam T, McElhinney A, Anders J, Rock TT, Lyker JK, Barcus S, Leslie KH, Kramer JM, Rubenstein EM, Schanz KP, Parkhurst AJ, Peck M, Good K, Granath KL, Cifra N, Detweiler JW, Stevens L, Albertson R, Deir R, Stewart E, Wingard K, Richardson MR, Blizard SB, Gillespie LE, Rzewnicki DI, Jones DH. 2016. T24 HRAS Transformed NIH/3T3 Mouse Cells (GhrasT-NIH/3T3) in Serial Tumorigenic Passages Give Rise to Increasingly Aggressive Tumorigenic Cell Lines T1-A and T2-A and Metastatic Cell Lines T3-HA and T4-PA. Experimental Cell Research 340 (1 – 11).
2015
RKR1/LTN1 UBIQUITIN LIGASE-MEDIATED DEGRADATION OF TRANSLATIONALLY STALLED ENDOPLASMIC RETICULUM PROTEINS
Crowder JJ, Geigges M, Gibson RT, Fults ES, Buchanan BW, Sachs N, Schink A, Kreft SG, Rubenstein EM. 2015. Rkr1/Ltn1 Ubiquitin Ligase-Mediated Degradation of Translationally Stalled Endoplasmic Reticulum Proteins. Journal of Biological Chemistry 290 (18454-18466).
2015
GROWTH-BASED DETERMINATION AND BIOCHEMICAL CONFIRMATION OF GENETIC REQUIREMENTS FOR PROTEIN DEGRADATION IN SACCHAROMYCES CEREVISIAE
Watts SG, Crowder JJ, Coffey SC, Rubenstein EM. 2015. Growth-based determination and biochemical confirmation of genetic requirements for protein degradation in Saccharomyces cerevisiae. Journal of Visualized Experiments 96 (e52428).
2013
N-TERMINAL ACETYLATION OF THE YEAST DERLIN DER1 IS ESSENTIAL FOR HRD1 UBIQUITIN-LIGASE ACTIVITY TOWARD LUMINAL ER SUBSTRATES
Zattas D, Adle DJ, Rubenstein EM, Hochstrasser M. N-terminal acetylation of the yeast Derlin Der1 is essential for Hrd1 ubiquitin-ligase activity toward luminal ER substrates. 2013. Molecular Biology of the Cell 24 (890-900).
2012
ABERRANT SUBSTRATE ENGAGEMENT OF THE SEC61 TRANSLOCON TRIGGERS DEGRADATION BY THE HRD1 UBIQUITIN LIGASE
Rubenstein EM, Kreft SG, Greenblatt W, Swanson RJ, Hochstrasser M. 2012. Aberrant substrate engagement of the Sec61 translocon triggers degradation by the Hrd1 ubiquitin ligase. Journal of Cell Biology 197, No. 6 (761-773).